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For adding the spot polygons to SFE objects converted from SPE.

Usage

addVisiumSpotPoly(x, spotDiameter)

Arguments

x

A SpatialFeatureExperiment object.

spotDiameter

Spot diameter for technologies with arrays of spots of fixed diameter per slide, such as Visium, ST, DBiT-seq, and slide-seq. The diameter must be in the same unit as the coordinates in the *Geometry arguments. Ignored for geometries that are not POINT or MULTIPOINT.

Value

A SFE object with a new colGeometry called spotPoly, which has polygons of the spots.

Examples

library(SpatialExperiment)
example(read10xVisium)
#> 
#> rd10xV> dir <- system.file(
#> rd10xV+   file.path("extdata", "10xVisium"), 
#> rd10xV+   package = "SpatialExperiment")
#> 
#> rd10xV> sample_ids <- c("section1", "section2")
#> 
#> rd10xV> samples <- file.path(dir, sample_ids, "outs")
#> 
#> rd10xV> list.files(samples[1])
#> [1] "raw_feature_bc_matrix" "spatial"              
#> 
#> rd10xV> list.files(file.path(samples[1], "spatial"))
#> [1] "scalefactors_json.json"    "tissue_lowres_image.png"  
#> [3] "tissue_positions_list.csv"
#> 
#> rd10xV> file.path(samples[1], "raw_feature_bc_matrix")
#> [1] "/home/runner/work/_temp/Library/SpatialExperiment/extdata/10xVisium/section1/outs/raw_feature_bc_matrix"
#> 
#> rd10xV> (spe <- read10xVisium(samples, sample_ids, 
#> rd10xV+   type = "sparse", data = "raw", 
#> rd10xV+   images = "lowres", load = FALSE))
#> class: SpatialExperiment 
#> dim: 50 99 
#> metadata(0):
#> assays(1): counts
#> rownames(50): ENSMUSG00000051951 ENSMUSG00000089699 ...
#>   ENSMUSG00000005886 ENSMUSG00000101476
#> rowData names(1): symbol
#> colnames(99): AAACAACGAATAGTTC-1 AAACAAGTATCTCCCA-1 ...
#>   AAAGTCGACCCTCAGT-1 AAAGTGCCATCAATTA-1
#> colData names(4): in_tissue array_row array_col sample_id
#> reducedDimNames(0):
#> mainExpName: NULL
#> altExpNames(0):
#> spatialCoords names(2) : pxl_col_in_fullres pxl_row_in_fullres
#> imgData names(4): sample_id image_id data scaleFactor
#> 
#> rd10xV> # base directory 'outs/' from Space Ranger can also be omitted
#> rd10xV> samples2 <- file.path(dir, sample_ids)
#> 
#> rd10xV> (spe2 <- read10xVisium(samples2, sample_ids, 
#> rd10xV+   type = "sparse", data = "raw", 
#> rd10xV+   images = "lowres", load = FALSE))
#> class: SpatialExperiment 
#> dim: 50 99 
#> metadata(0):
#> assays(1): counts
#> rownames(50): ENSMUSG00000051951 ENSMUSG00000089699 ...
#>   ENSMUSG00000005886 ENSMUSG00000101476
#> rowData names(1): symbol
#> colnames(99): AAACAACGAATAGTTC-1 AAACAAGTATCTCCCA-1 ...
#>   AAAGTCGACCCTCAGT-1 AAAGTGCCATCAATTA-1
#> colData names(4): in_tissue array_row array_col sample_id
#> reducedDimNames(0):
#> mainExpName: NULL
#> altExpNames(0):
#> spatialCoords names(2) : pxl_col_in_fullres pxl_row_in_fullres
#> imgData names(4): sample_id image_id data scaleFactor
#> 
#> rd10xV> # tabulate number of spots mapped to tissue
#> rd10xV> cd <- colData(spe)
#> 
#> rd10xV> table(
#> rd10xV+   in_tissue = cd$in_tissue, 
#> rd10xV+   sample_id = cd$sample_id)
#>          sample_id
#> in_tissue section1 section2
#>     FALSE       28       27
#>     TRUE        22       22
#> 
#> rd10xV> # view available images
#> rd10xV> imgData(spe)
#> DataFrame with 2 rows and 4 columns
#>     sample_id    image_id   data scaleFactor
#>   <character> <character> <list>   <numeric>
#> 1    section1      lowres   ####   0.0510334
#> 2    section2      lowres   ####   0.0510334
# There can't be suplicate barcodes
colnames(spe) <- make.unique(colnames(spe), sep = "-")
rownames(spatialCoords(spe)) <- colnames(spe)
sfe <- toSpatialFeatureExperiment(spe)
# A hypothetical spot diameter; check the scalefactors_json.json file for
# actual diameter in pixels in full resolution image.
sfe <- addVisiumSpotPoly(sfe, spotDiameter = 80)